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Briefings in Bioinformatics Nov 2019Precision medicine is rapidly emerging as a strategy to tailor medical treatment to a small group or even individual patients based on their genetics, environment and... (Review)
Review
Precision medicine is rapidly emerging as a strategy to tailor medical treatment to a small group or even individual patients based on their genetics, environment and lifestyle. Precision medicine relies heavily on developments in systems biology and omics disciplines, including metabolomics. Combination of metabolomics with sophisticated bioinformatics analysis and mathematical modeling has an extreme power to provide a metabolic snapshot of the patient over the course of disease and treatment or classifying patients into subpopulations and subgroups requiring individual medical treatment. Although a powerful approach, metabolomics have certain limitations in technology and bioinformatics. We will review various aspects of metabolomics technology and bioinformatics, from data generation, bioinformatics analysis, data fusion and mathematical modeling to data management, in the context of precision medicine.
Topics: Computational Biology; Humans; Metabolomics; Precision Medicine; Tissue Banks
PubMed: 29304189
DOI: 10.1093/bib/bbx170 -
Methods in Molecular Biology (Clifton,... 2019A biobank is an important nexus between clinical and research aspects of pathology. The collection and storage of high quality surgical samples is essential for... (Review)
Review
A biobank is an important nexus between clinical and research aspects of pathology. The collection and storage of high quality surgical samples is essential for diagnosis post-surgery, and can also be used to create vaccines, identify therapeutic targets or establish eligibility of cancer patients in a clinical trial. Therefore, personnel handling surgical tissues should follow standard operating procedures (SOP) to maximize efficiency and preserve tissue quality. This chapter is intended to familiarize novice biobank personnel with the issues associated with different steps of surgical tissue collection including patient consent, sample collection, tissue storage, quality control, and distribution.
Topics: Biological Specimen Banks; Humans; Neoplasms; Quality Control; Specimen Handling; Tissue Banks
PubMed: 30539435
DOI: 10.1007/978-1-4939-8935-5_7 -
Cryobiology Dec 2015We describe here a new cryobiological and neurobiological technique, aldehyde-stabilized cryopreservation (ASC), which demonstrates the relevance and utility of advanced...
We describe here a new cryobiological and neurobiological technique, aldehyde-stabilized cryopreservation (ASC), which demonstrates the relevance and utility of advanced cryopreservation science for the neurobiological research community. ASC is a new brain-banking technique designed to facilitate neuroanatomic research such as connectomics research, and has the unique ability to combine stable long term ice-free sample storage with excellent anatomical resolution. To demonstrate the feasibility of ASC, we perfuse-fixed rabbit and pig brains with a glutaraldehyde-based fixative, then slowly perfused increasing concentrations of ethylene glycol over several hours in a manner similar to techniques used for whole organ cryopreservation. Once 65% w/v ethylene glycol was reached, we vitrified brains at -135 °C for indefinite long-term storage. Vitrified brains were rewarmed and the cryoprotectant removed either by perfusion or gradual diffusion from brain slices. We evaluated ASC-processed brains by electron microscopy of multiple regions across the whole brain and by Focused Ion Beam Milling and Scanning Electron Microscopy (FIB-SEM) imaging of selected brain volumes. Preservation was uniformly excellent: processes were easily traceable and synapses were crisp in both species. Aldehyde-stabilized cryopreservation has many advantages over other brain-banking techniques: chemicals are delivered via perfusion, which enables easy scaling to brains of any size; vitrification ensures that the ultrastructure of the brain will not degrade even over very long storage times; and the cryoprotectant can be removed, yielding a perfusable aldehyde-preserved brain which is suitable for a wide variety of brain assays.
Topics: Animals; Brain; Cryopreservation; Cryoprotective Agents; Ethylene Glycol; Glutaral; Rabbits; Swine; Tissue Banks; Vitrification
PubMed: 26408851
DOI: 10.1016/j.cryobiol.2015.09.003 -
Seminars in Oncology Oct 2015Personalized oncology, or more aptly precision oncogenomics, refers to the identification and implementation of clinically actionable targets tailored to an individual... (Review)
Review
Personalized oncology, or more aptly precision oncogenomics, refers to the identification and implementation of clinically actionable targets tailored to an individual patient's cancer genomic information. Banking of human tissue and other biospecimens establishes a framework to extract and collect the data essential to our understanding of disease pathogenesis and treatment. Cancer cooperative groups in the United States have led the way in establishing robust biospecimen collection mechanisms to facilitate translational research, and combined with technological advances in molecular testing, tissue banking has expanded from its traditional base in academic research and is assuming an increasingly pivotal role in directing the clinical care of cancer patients. Comprehensive screening of tumors by DNA sequencing and the ability to mine and interpret these large data sets from well-organized tissue banks have defined molecular subtypes of cancer. Such stratification by genomic criteria has revolutionized our perspectives on cancer diagnosis and treatment, offering insight into prognosis, progression, and susceptibility or resistance to known therapeutic agents. In turn, this has enabled clinicians to offer treatments tailored to patients that can greatly improve their chances of survival. Unique challenges and opportunities accompany the rapidly evolving interplay between tissue banking and genomic sequencing, and are the driving forces underlying the revolution in precision medicine. Molecular testing and precision medicine clinical trials are now becoming the major thrust behind the cooperative groups' clinical research efforts.
Topics: Biomedical Research; Cooperative Behavior; Data Mining; Genetic Testing; Genomics; High-Throughput Nucleotide Sequencing; Humans; Medical Oncology; Neoplasms; Precision Medicine; Sequence Analysis, DNA; Specimen Handling; Tissue Banks
PubMed: 26433552
DOI: 10.1053/j.seminoncol.2015.07.013 -
Cell and Tissue Banking Dec 2016For successful transplantation, allografts should be free of microorganisms that may cause harm to the allograft recipient. Before or during recovery and subsequent... (Review)
Review
For successful transplantation, allografts should be free of microorganisms that may cause harm to the allograft recipient. Before or during recovery and subsequent processing, tissues can become contaminated. Effective tissue recovery methods, such as minimizing recovery times (<24 h after death) and the number of experienced personnel performing recovery, are examples of factors that can affect the rate of tissue contamination at recovery. Additional factors, such as minimizing the time after asystole to recovery and the total time it takes to perform recovery, the type of recovery site, the efficacy of the skin prep performed immediately prior to recovery of tissue, and certain technical recovery procedures may also result in control of the rate of contamination. Due to the heterogeneity of reported recovery practices and experiences, it cannot be concluded if the use of other barriers and/or hygienic precautions to avoid contamination have had an effect on bioburden detected after tissue recovery. Qualified studies are lacking which indicates a need exists for evidence-based data to support methods that reduce or control bioburden.
Topics: Allografts; Cell Culture Techniques; Decontamination; Humans; Specimen Handling; Sterilization; Tissue Banks; Transplantation, Homologous
PubMed: 27761677
DOI: 10.1007/s10561-016-9590-5 -
Journal of Applied Biomaterials &... Nov 2016Bioencapsulation technologies have played an important role in the developing successes of tissue engineering. Besides offering immunoisolation, they also show promise... (Review)
Review
Bioencapsulation technologies have played an important role in the developing successes of tissue engineering. Besides offering immunoisolation, they also show promise for cell/tissue banking and the directed differentiation of stem cells, by providing a unique microenvironment. This review describes bioencapsulation technologies and summarizes their recent progress in research into tissue engineering. The review concludes with a brief outlook regarding future research directions in this field.
Topics: Animals; Capsules; Humans; Tissue Banks; Tissue Engineering
PubMed: 27716872
DOI: 10.5301/jabfm.5000299 -
Croatian Medical Journal Jun 2021
Topics: Cryopreservation; Humans; Tissue Banks
PubMed: 34212567
DOI: 10.3325/cmj.2021.62.297 -
Experimental Biology and Medicine... Dec 2018Sterility testing is a critical issue in the recovery, processing, and release of tissue allografts. Contaminated allografts are often discarded, increasing costs, and... (Review)
Review
Sterility testing is a critical issue in the recovery, processing, and release of tissue allografts. Contaminated allografts are often discarded, increasing costs, and reducing tissue stocks. Given these concerns, it is important to determine the most effective methodology for sterility testing. This work provides an overview of microbiological methods for sampling and culturing donor grafts for cardiovascular tissue banking.
Topics: Cardiovascular System; Decontamination; Humans; Tissue Banks
PubMed: 30614255
DOI: 10.1177/1535370218820515 -
Frontiers in Endocrinology 2022Traditional fertility preservation methods such as embryo or oocyte cryopreservation cannot meet the needs of a cancer patient or for personal reasons. The... (Review)
Review
Traditional fertility preservation methods such as embryo or oocyte cryopreservation cannot meet the needs of a cancer patient or for personal reasons. The cryopreservation of ovarian tissue can be an alternative and has become a hot spot to preserve fertility or hormone replacement. The freezing of ovarian tissue can be carried out at any time without ovarian hyperstimulation to retrieve follicles. It is an ideal strategy to preserve reproductive function in children, adolescent cancer patients, and patients who are in urgent need of cancer treatment. With the increasing demands of women with premature ovarian failure or in menopause, ovarian tissue transplantation is also an alternative for hormone replacement that can provide physiological doses of hormone levels, which can avoid a series of risks such as thrombosis, breast cancer, or other hormone-dependent tumors, caused by oral hormone replacement. Hence, ovarian tissue banking can be regarded as a mainstream strategy for fertility preservation and anti-menopause hormone replacement in further clinical investigation.
Topics: Breast Neoplasms; Female; Fertility Preservation; Hormones; Humans; Ovary; Tissue Banks
PubMed: 36157465
DOI: 10.3389/fendo.2022.950297 -
Annals of Transplantation 2004Historical remarks and data concerning the present status of tissue preservation and storage are presented. Even though, the need for preserved tissue allografts is... (Review)
Review
Historical remarks and data concerning the present status of tissue preservation and storage are presented. Even though, the need for preserved tissue allografts is still very high and tissue bank services are very popular, the opinion is expressed that the future of tissue banking may be correlated with organ and tissue engineering.
Topics: Animals; Humans; Organ Transplantation; Stem Cells; Tissue Banks; Tissue Engineering; Tissue Preservation
PubMed: 15478902
DOI: No ID Found